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鎶�琛�(sh霉)鏂囩嵒
涓�銆佸椹�?z膩i)搴峰憪c鍘熺悊
铔嬬櫧璩�(zh矛)鍦ㄨ仛涓欑儻閰拌兒鍑濊啝涓浕娉虫檪锛屽畠鐨勯伔绉诲彇姹轰簬瀹冩墍甯堕浕鑽蜂互鍙婂垎瀛愬ぇ灏忓拰褰㈢媭绛夊洜绱�銆�1967骞碨hapiro绛変汉鐧�(f膩)鐝�(xi脿n)锛屽鏋滃湪鑱氫笝鐑叞鑳虹郴绲�(t菕ng)涓姞鍏ラ櫚闆㈠瓙鍘绘薄鍔戝崄浜岀兎鍩虹：閰搁垑锛圫DS锛�锛屽ぇ澶氭暩(sh霉)铔嬬櫧璩�(zh矛)鑳借垏SDS鎸変竴瀹氭瘮渚嬬祼(ji茅)鍚�锛屽嵆姣忓厠铔嬬櫧璩�(zh矛)绲�(ji茅)鍚�1.4g鐨凷DS锛嶅京(f霉)鍚堢墿閮藉付涓婄浉鍚屽瘑搴︾殑璨�(f霉)闆昏嵎锛屽畠鐨勯噺澶уぇ瓒呴亷浜嗚泲鐧借唱(zh矛)鍒嗗瓙鍘熸湁鐨勯浕鑽烽噺锛屽洜鑰屾秷闄や簡铔嬬櫧璩�(zh矛)鍘熸湁鐨勯浕鑽峰樊鍒ワ紝浣胯泲鐧借唱(zh矛)鍒嗗瓙闆绘吵鐨勯伔绉荤巼涓昏鍙栨焙浜庢湰韬殑鍒嗗瓙閲�锛岃�岃垏铔嬬櫧璩�(zh矛)鎵�甯剁殑闆昏嵎鐒￠棞(gu膩n)锛屽湪涓�瀹氭浠朵笅锛岃泲鐧借唱(zh矛)鐨勫垎瀛愰噺鐨勫皪鏁�(sh霉)鑸囬浕娉抽伔绉荤巼闁撳憟璨�(f霉)鐩搁棞(gu膩n)銆�
鏈椹楃殑鐩殑鏄皪澶氶厷姘у寲閰剁殑绱斿寲搴﹂憭瀹氬強鍒嗗瓙閲忕殑娓畾锛岄�氶亷瀵﹂锛屽(xu茅)缈�(x铆)鍜屾帉鎻DS鑱氫笝鐑叞鑳哄嚌鑶犻浕娉虫硶铔嬬櫧璩�(zh矛)绱斿害鍜屽垎瀛愰噺鐨勯憭瀹�銆�
浜�銆佸剙鍣ㄨ垏瑭﹀姂
1銆佹潗鏂欙細
纭吀閵ㄩ菇鏋愭矇娣�鐨勫閰氭哀鍖栭叾绮楅叾妯ｅ搧銆丏EAE-绾栫董绱燚E52鏌卞堡鏋愮殑妯ｅ搧锛孲ephadex G-100鏌卞堡鏋愮殑妯ｅ搧銆�
2銆佽│鍔戯細
锛�1锛変笝绋�閰拌兒锛圓cr姣嶆恫锛夛細30%Acr 锛圓cr/Bis锛�
锛�2锛�10%鐨凷DS婧舵恫
锛�3锛�10%鐨勯亷纭吀閵ㄦ憾娑�
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锛�5锛夊垎闆㈣啝绶╂矕娑诧細1.5M Tris锛孭H8.8
锛�6锛夋績绺啝绶╂矕娑诧細1.0M Tris锛孭H6.8
锛�7锛夐浕妤电珐娌栨恫锛�10×30g Tris锛�125g 鐢樻皑閰稿拰5g SDS锛屽姞姘存憾瑙ｅ畾瀹硅嚦1000ml锛宲H8.3
锛�8锛夋ǎ鍝佺珐娌栨恫锛�0.2M Tris锛孭H6.8锛�1%SDS锛�30%鐢樻补锛屽钒鍩轰箼閱囧強婧撮厷铇�
锛�9锛夋煋鑹叉恫锛�0.15%鑰冮Μ鏂寒钘�(l谩n)R250锛屾憾浜庤劔鑹叉恫
锛�10锛夎劔鑹叉恫锛�50%鐨勭敳閱�锛�7%鐨勫啺閱嬮吀鐨勬按婧舵恫
锛�11锛夋(bi膩o)婧�(zh菙n)鍒嗗瓙閲忚泲鐧�銆�
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鐢ㄥ叐濉婇浕娉崇幓鐠冩澘鍒舵垚鍨傜洿鏉挎Ы锛堜笉鑳芥紡鑶狅級锛屽瀭鐩存斁缃�銆傚皣閰嶅埗濂界殑鍒嗛洟鑶犳憾娑插�掑叆锛屾淮鍔犲叆鐒￠洟瀛愭按锛屽緟鍑濊啝鑱氶泦鍚庯紝鍊掑嚭鐒￠洟瀛愭按锛岀敤鍚告按绱欏惛骞�锛屽�掑叆婵冪府鑶�锛屽啀鎻掑叆姊冲瓙銆�
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鍒嗗垾鍙栨ǎ鍝佽嫢骞瞞l浜庨洟蹇冪涓紝鎸�1/1锝�1/5姣斾緥鍔犲叆5×妯ｅ搧绶╂矕娑�锛屽啀娌告按娴翠腑鍔犵啽3锝�5min锛屽彇鍑哄緟鐢�銆傜敤寰噺娉ㄥ皠鍣ㄥ垎鍒ュ惛鍙栦笉瓒呴亷 30μl涓嶅悓婵冨害鐨勬(bi膩o)婧�(zh菙n)铔嬬櫧妯ｅ搧鍜岃│椹楁ǎ鍝佹敞鍏ユǎ鍝佹Ы銆傞粸妯ｇ祼(ji茅)鏉熷悗锛岃(di脿o)绡�(ji茅)闆绘吵鍎�闆绘祦鍒�10mA锛�2锝�3mA/em锛�锛屼繚鎸侀浕娴佺┅(w臎n)瀹氫笉璁�锛岀暥(d膩ng)婧撮厷钘�(l谩n)閬风Щ鍒伴洟鍒嗛洟鑶犲簳1锝�2cm鏅�锛屽嵆鍙仠姝㈤浕娉�銆�
3銆佹煋鑹诧細
闆绘吵瀹岀暍鍚�锛屽彇鍑哄嚌鑶犳澘锛屾蹈鍏ユ煋鑹叉恫涓�锛屽湪37鈩冩韩绠变腑淇濇韩閬庡銆傚�掓帀鏌撹壊娑�锛�24h鍚�锛屽嵆鍙湅鍒版竻鏅扮殑铔嬬櫧璩�(zh矛)姊濆付銆�
鍥涖�佺祼(ji茅)鏋� 锛堢暐锛�
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4銆佹湁鐨勮泲鐧借唱(zh矛)锛堝锛氶浕鑽风暟甯告垨绲�(ji茅)妲�(g貌u)鐣板父鐨勮泲鐧借唱(zh矛)锛涘付鏈夎純澶ц紨鍩虹殑铔嬬櫧璩�(zh矛)锛変笉鑳介噰鐢ㄨ┎娉曟脯鐩稿皪鍒嗗瓙閲忋��
5銆佸鏋滆┎闆绘吵涓嚭鐝�(xi脿n)鎷栧熬銆佹煋鑹插付鐨勮儗鏅笉娓呮櫚绛夌従(xi脿n)璞�锛屽彲鑳芥槸SDS涓嶇磾寮曡捣銆�
